Bioanalytical Techniques focuses on new as well as conventional bioanalytical and biophysical tools, emerging techniques, and instruments and probes the molecular aspects of biological systems. The primary aim is to provide the reader with the fundamentals and basic techniques such as sensors, separations, mass spectrometry, spectroscopy, arrays, microfluidics, etc. Biophysical techniques such as magnetic resonance; optical, electron and x-ray microscopy; x-ray and neutron techniques have also been introduced. Bioanalytical Techniques offer vast possibilities to examine and explore biological systems (for example, proteomics, genomics, metabolomics, sequencing, screening, characterizing macromolecular interactions, or clinical applications) both in vivo and in vitro. Therefore, various DNA techniques are given in sufficient detail for the students as well as professionals.

Preface / Introduction / Section-1: General Principles and Techniques – Microscopy / Aqueous Solutions / Spectroscopy / Centrifugation / Chromatography / Electrophoresis / Radioisotopic Techniques / Section-2: Enzyme and Proteins – Protein Purification / Structure of Proteins / Peptide Sequencing / Mapping and Synthesis / Enzyme Kinetics / Immobilized Enzymes / Section-3: Immunological Analysis – Antigen and Antibody Techniques / Section-4: DNA Techniques – Nucleic Acid / DNA / RNA / DNA Modifying Enzymes / Immunochemical Assays / Immunology / PCR Techniques / Section-5: Cell Technology – Eukaryotic Cells / Fermentation Process / Section-6: Genetic Engineering – Genetic Engineering / Tools of Genetic Engineering / Techniques of Genetic Engineering / Genetic Engineering for Human Health / Glossary / Index.

Undergraduate and Postgraduate Students